Preview

Obstetrics, Gynecology and Reproduction

Advanced search

Magnesium-containing composition influencing morphological parameters and gelatinase B activity in endometrial tissues in experimental chronic endometrial inflammation

https://doi.org/10.17749/2313-7347/ob.gyn.rep.2020.143

Full Text:

Abstract

Aim: to assess an effect of medicinal magnesium-containing composition on gelatinase B expression intensity and morphological parameters of chronic experimental endometrial inflammation.

Materials and Methods. There were conducted experiments with 60 sexually mature female Wistar rats to determine an effect of medicinal magnesium-containing composition (contains magnesium chloride – a natural polymineral Bischofite) on gelatinase B activity in endometrial tissues (counting number of gelatinase-B-positive cells and intensity of gelatinase B expression), morphological parameters of cell infiltration, as well as amount of magnesium in red blood cell mass collected from the inferior vena cava and subclavian vein. All such parameters were assessed in experimental animals from 4 groups: group 1 – animals in baseline state, group 2 – control, group 3 – experimental chronic endometritis (CE), group 4 – experimental CE after treatment with medicinal magnesium-containing composition.
Results. It was found that use of medicinal magnesium-containing composition increased amount of erythrocyte magnesium up to the baseline level and increased both number and expression intensity of stromal gelatinase B-positive cells. In addition, magnesium level in erythrocyte mass from the inferior vena cava was increased and accompanied with restored eosinophil-plasmacyte as well as significantly elevated macrophage-lymphocyte infiltration in endometrial tissues compared to CE animals lacking therapy.
Conclusion. The results of this study allow us to conclude about importance of gelatinase B in pathogenesis of experimental chronic endometrial inflammation as well as an opportunity of regulating gelatinase B activity by applying medicinal magnesium containing drug in pathogenetic therapy of experimental CE.

About the Authors

L. N. Rogova
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Lyudmila N. Rogova – MD, Dr Sci Med, Professor, Head of the Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131



K. Yu. Tikhaeva
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Kseniya Yu. Tikhaeva – MD, PhD, Associate Professor, Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131



L. V. Tkachenko
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Lyudmila V. Tkachenko – MD, Dr Sci Med, Professor, Head of the Department of Obstetrics and Gynecology

1 Pavshikh Bortsov Sq., Volgograd 400131



N. V. Shesternina
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Nataliya V. Shesternina – MD, PhD, Associate Professor, Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131

 


V. N. Povetkina
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Victoria N. Povetkina – MD, PhD, Associate Professor, Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131



V. A. Staravoitov
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Vladislav A. Staravoitov – MD, PhD, Associate Professor, Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131



S. Yu. Dyachkova
Volgograd State Medical University, Health Ministry of Russian Federation
Russian Federation

Svetlana Yu. Dyachkova – MD, PhD, Associate Professor, Department of Pathophysiology, Clinical Pathophysiology

1 Pavshikh Bortsov Sq., Volgograd 400131



References

1. Cicinelli E., Matteo M., Tinelli R. et al. Prevalence of chronic endometritis in repeated unexplained implantation failure and the IVF success rate after antibiotic therapy. Hum Reprod. 2015;30(2):323–30. https://doi.org/10.1093/humrep/deu292.

2. Johnston-MacAnanny E.B., Hartnett J., Engmann L.L. et al. Chronic endometritis is a frequent finding in women with recurrent implantation failure after in vitro fertilization. Fertil Steril. 2010;93(2):437–41. https://doi.org/10.1016/j.fertnstert.2008.12.131.

3. Park H.J., Kim Y.S., Yoon T.K., Lee W.S. Chronic endometritis and infertility. Clin Exp Reprod Med. 2016;43(4):185–92. https://doi.org/10.5653/cerm.2016.43.4.185.

4. Sukhikh G.T., Shurshalina A.V. Chronic endometritis. Guideline. [Hronicheskij endometrit. Rukovodstvo.]. Moskva: GEOTAR-Media, 2010. 64 s. (In Russ.).

5. Extracellular matrix: new perspectives for translational medicine. Matrix Biology. 2016;49:144.

6. 6. Krieg T., Aumailley M. The extracellular matrix of the dermis: flexible structures with dynamic functions. Exp Dermatol. 2011;20(8):689–95. https://doi.org/10.1111/j.1600-0625.2011.01313.x.

7. Pereza N., Ostojić S., Volk M. et al. Matrix metalloproteinases 1, 2, 3 and 9 functional single-nucleotide polymorphisms in idiopathic recurrent spontaneous abortion. Reprod Biomed Online. 2012;24(5):567–75. https://doi.org/10.1016/j.rbmo.2012.01.008.

8. Baker A.H., Edwards D.R., Murphy G. Metalloproteinase inhibitors: biological actions and therapeutic opportunities. J Cell Science. 2002;115(Pt 19):3719–27. https://doi.org/10.1242/jcs.00063.

9. Wallach J. Matrix metalloproteinases and cancer. Novel perspectives in the role and control of matrix metalloproteinases in tumor progression. Biochimie. 2005;87(3–4):241. https://doi.org/10.1016/j.biochi.2205.05.001.

10. Rogova L.N., Shesternina N.V., Zamechnik T.V., Fastova I.A. Matrix metalloproteinases, their role in physiological and pathological processes (review). [Matriksnye metalloproteinazy, ih rol' v fiziologicheskih i patologicheskih processah (obzor)]. Vestnik novyh medicinskih tekhnologij. 2011;18(2):86–9. (In Russ.).

11. Poteryaeva O.N. Matrix metalloproteinases: structure, regulation, role in the development of pathological states (literature review). [Matriksnye metalloproteinazy: stroenie, regulyaciya, rol' v razvitii patologicheskih sostoyanij (obzor literatury)]. Medicina i obrazovanie v Sibiri. 2010;(5). (In Russ.). Available at: cyberleninka.ru/article/n/matriksnye-metalloproteinazy-stroenie-regulyatsiya-rol-v-razvitiipatologicheskih-sostoyaniy-obzor-literatury. [Accessed: 04.05.2020].

12. Rogova L.N., Shesternina N.V., Staravoytov V.A. The influence of the magnesium-containing composition on magnesium balance, intensity peroxidation and activity of antioxidant enzymes in rats with an acidinduced gastric ulcer. [Vliyanie magnijsoderzhashchej kompozicii na magnievyj balans, intensivnost' peroksidacii i aktivnost' antioksidantnyh fermentov u krys s acetatnoj yazvoj zheludka]. Vestnik novyh medicinskih tekhnologij. 2011;18(2):89–91. (In Russ.).

13. Torshin I.Yu., Gromova O.A. Magnesium and pyridoxine: fundamental studies and clinical practice. New York: Nova Science Publishers, 2011. 196 p.

14. Tikhaeva K.Yu., Tkachenko L.V., Rogova L.N., Kurkin D.V. A method of modeling chronic endometrial inflammation and its pathogenetic substantiation. [Metod modelirovaniya hronicheskogo vospaleniya endometriya i ego patogeneticheskoe obosnovanie]. Volgogradskij nauchno-medicinskij zhurnal. 2015;(1):31–4. (In Russ.).

15. Ganicheva L.M. Methodological aspects of developing drugs based on natural mineral bishofit. [Metodologicheskie aspekty razrabotki lekarstvennyh preparatov na osnove prirodnogo poliminerala bishofit]. Vestnik Volgogradskogo gosudarstvennogo medicinskogo universiteta. 2007;(1):69–73. (In Russ.).

16. Alimova O.A., Voropaeva E.E., Kazachkova E.A., Kazachkov E.L. Semiquantitative morphological assessment of inflammatory process activity in chronic endometritis. [Polukolichestvennaya morfologicheskaya ocenka aktivnosti vospalitel'nogo processa pri hronicheskom endometrite. Vserossijskaya nauchno-prakticheskaya konferenciya «Aktual'nye problemy patologoanatomicheskoj sluzhby municipal'nyh uchrezhdenij zdravoohraneniya»]. Chelyabinsk, 2008. 198–201. (In Russ.).


For citation:


Rogova L.N., Tikhaeva K.Yu., Tkachenko L.V., Shesternina N.V., Povetkina V.N., Staravoitov V.A., Dyachkova S.Yu. Magnesium-containing composition influencing morphological parameters and gelatinase B activity in endometrial tissues in experimental chronic endometrial inflammation. Obstetrics, Gynecology and Reproduction. 2020;14(6):637-645. (In Russ.) https://doi.org/10.17749/2313-7347/ob.gyn.rep.2020.143

Views: 160


ISSN 2313-7347 (Print)
ISSN 2500-3194 (Online)