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The rs1940475 polymorphism in the MMP8 gene as a protective factor of severe breast cancer


Aim: to investigate an association between polymorphism in the MMP2, MMP8, MMP9 genes and severe breast cancer (BC).

Materials and Methods. Retrospective comparative study was conducted by assessing a cohort of 345 BC patients: 254 patients at stage I–II and 91 at stage III–IV. Genotyping of the three single nucleotide polymorphisms in the MMP2 (C>T rs243865), MMP8 C>T rs1940475), and ММР9 (C>T rs3918242) genes was performed. A comparative analysis of the genetic characteristics of patients with stage I–II and stage III–IV of the disease was performed.

Results. The allele variant T rs1940475 (C>T) of the MMP8 gene in BC patients at stage I–II (0.512) was found by 1.2-fold more often compared with patients at stage III–IV of the disease (0.428; pperm = 0.05). According to allelic (odds ratio (OR) = 0.71; 95 % confidence interval (CI) = 0.51–1.00; pperm = 0.05) and additive (OR = 0.69; 95 % CI = 0.48–0.99; pperm = 0.05) genetic models, the allele T rs1940475 (C>T) of the MMP8 gene has protective effect for developing stage III–IV BC. The polymorphic locus rs1940475 (C>T) determines the amino acid substitution in the MMP8 protein (p.K87E) and DNA binding to the transcription factors NF-AT1, MYC and CIZ, is associated with the expression of the genes MMP27 and RP11-817J15.3 acting as an enhancer regulatory site in primary breast epithelial cells. Single nucleotide polymorphism in the MMP2 (C>T rs243865), ММР9 (C>T rs3918242) genes is not associated with severe BC (stages III–IV).

Conclusion. The single nucleotide polymorphism rs1940475 in the MMP8 gene is a protective factor protecting against severe BC course.

About the Authors

N. V. Pavlova
Belgorod Regional Oncological Dispensary
Russian Federation

Nadezhda V. Pavlova – MD, Head of the Polyclinic Department

1 Kuibyshev Str., Belgorod 308010, Russia

I. V. Ponomarenko
Belgorod State National Research University
Russian Federation

Irina V. Ponomarenko – MD, Dr Sci Med, Professor, Department of Biomedical Disciplines

85 Pobedy Str., Belgorod 308007, Russia

M. I. Churnosov
Belgorod State National Research University
Russian Federation

Mikhail I. Churnosov – MD, Dr Sci Med, Professor, Head of the Department of Biomedical Disciplines

85 Pobedy Str., Belgorod 308007, Russia


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For citations:

Pavlova N.V., Ponomarenko I.V., Churnosov M.I. The rs1940475 polymorphism in the MMP8 gene as a protective factor of severe breast cancer. Obstetrics, Gynecology and Reproduction. 2022;16(4):401-409. (In Russ.)

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