PROTEIN MARKERS OF THE HYPOXIC-ISCHEMIC INJURY OF THE CENTRAL NERVOUS SYSTEM IN THE PERINATAL PERIOD
Abstract
Preeclampsia (gestosis) and eclampsia, as well as the acute intranatal hypoxy often act as trigger for the development of the perinatal hypoxic-ischemic brain injury. That is accompanied by such typical structural changes as periventricular leukomalacia (PVL) and intraventricular hemorrhages (IVH). The hypoxic-ischemic brain injury in the perinatal period can have such consequences as hydrocephaly, microcephaly, infantile cerebral paralysis (ICP), epilepsy and motormental retardation. It is not always possible to determine the disease gravity and forecast of the disease by routine methods of clinical, instrumental and laboratory examination. As it is proved, that the perinatal hypoxic-ischemic brain injury is always accompanied by the disruption of Blood-Brain-Barrier (BBB) permeability for neuron-specific proteins (NSP). NSP can be considered as markers of this pathologic process. Nowadays there are more or less details on 120 NSP, namely, non-enzyme neurospecific Са+ -binding proteins; non-enzyme NSP, responsible for adhesion and intercellular recognition processes; соntractile and cytoskeletal nervous tissue proteins; secreted regulatory and transport NSP; myeline proteins and glial NSP. To evaluate BBB status it is expedient to select from the variety of known NSP most studied proteins, being neurons’ and astrocytes’ markers. Such NSP are gliofibrillary acid protein, GFAP and neuronspecific enolase, NSE. In normal conditions the NSP are within BBB and can be almost undeterminable in the blood serum. In case of the BBB permeability disruption NSP penetrate to the peripheral blood flow and can be determined. The dynamic determination of the NSP in the blood serum can be expedient to evaluate the BBB resistance, to determine the degree of the CNS injury and the disease state forecast at children with perinatal hypoxic-ischemic brain injury.
About the Author
D. V. Blinov
The Russian National Research Medical University named after N.I. Pirogov of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
PhD, Pirogov Russian National Research Medical University (RNRMU),
ul. Ostrovitianova, 1, Moscow, 117997
References
1. Ashmarin I.P., Stukalov P.V. Neirokhimiya. 1996; 470 s.
2. Blinov D.V. Akusherstvo, ginekologiya i reproduktsiya / Obstetrics, gynecology and reproduction. 2011; 2: 5-12.
3. Blinov D.V. Epilepsiya i paroksizmal'nye sostoyaniya / Epilepsy and paroxysmal conditions. 2011; 2: 28-33
4. Blinov D.V. Enzyme immunoassay of neuron-specific antigens in evaluation of blood-brain-barrier permeability in perinatal hypoxic-ischemic lesion of CNS (clinical and experimental study). PhD Thesis. [Immunofermentnyi analiz neirospetsificheskikh antigenov v otsenke pronitsaemosti gematoentsefalicheskogo bar'era pri perinatal'nom gipoksicheskiishemicheskom porazhenii TsNS (kliniko-eksperimental'noe issledovanie) Diss. …kand. med. nauk (in Russian)]. Moscow. 2004; 153 s.
5. Blinov D.V., Sandukovskaya S.I. Epilepsiya i paroksizmal'nye sostoyaniya / Epilepsy and paroxysmal conditions. 2010; 2 (4): 12-22.
6. Bredberi M. The concept of blood-brain barrier [Kontseptsiya gematoentsefalicheskogo bar'era (in Russian)]. Moscow. 1983; 480 s.
7. Gurina O.I., Dmitrieva T.B., Lebedev S.V., Blinov D.V., Ryabukhin I.A., Petrov S.V., Semenova A.V., Rogatkin S.O., Volodin N.N., Chekhonin V.P. Chapter in the book: Chekhonin V.P., Gurina O.I., Dmitrieva T.B. Monoclonal antibodies to proteins neurospecific [Monoklonal'nye antitela k neirospetsificheskim belkam (in Russian)]. Moscow. 2007; 344 s.
8. Classification of the effects of perinatal lesions of the nervous system in newborns. Guidelines [Klassifikatsiya posledstvii perinatal'nykh porazhenii nervnoi sistemy u novorozhdennykh. Metodicheskie rekomendatsii]. Moscow. 2005; 40 s.
9. Mukhtarova S.N. Meditsinskie Novosti Gruzii. 2010; 4 (181): 49-54.
10. Order of the Health Ministry of Russia № 1687n from December 27, 2011 [Prikaz Minzdravsotsrazvitiya Rossii № 1687n ot 27 dekabrya 2011 g. (in Russian)]
11. Chekhonin V.P., Dmitrieva T.B., Zhirkov Yu.A. Immunochemical analysis of antigens neurospecifical [Immunokhimicheskii analiz neirospetsificheskikh antigenov (in Russian)]. Moscow. 2000; 416 s.
12. Chekhonin V.P., Lebedev S.V., Blinov D.V., Gurina O.I., Semenova A.V., Lazarenko I.P., Petrov S.V., Ryabukhin I.A., Rogatkin S.O., Volodin N.N. Voprosy ginekologii, akusherstva i perinatologii. 2004; 3 (2) 50-56.
13. Yakunin Yu.A., Perminov V.S. Ros. Vest. perinat. i ped. 1993; 38 (2): 20-24.
14. Berger R., Pierce M., Wisnievski S., Adelson P., Clark R., Ruppel R., Kochanek P. Pediatrics. 2002; 109 (2): 34-38.
15. Blennow M., Savman K., Ilves P., Thoresen M., Rosengren L. Acta Paediatr. 2001; 90: 1171-1175.
16. Clark R., Kochalek P., Adelson P. J. Pediatr. 2000; 137: 197-204.
17. Di Domenico F., Coccia R., Butterfield D.A., Perluigi M. Biochimica Et Biophysica Acta. 2011; 1814 (12): 1785-1795.
18. Einav S., Kaufman N., Algur N., Kark J.D. J Am Coll Cardiol. 2012; 60 (4): 304-311.
19. Elimian A., Figueroa R., Verma U., Visintainer P., Sehgal P, Tejani N. Obst. Gynecol. 1998; 92 (1): 546-55.
20. Eng L.F., Ghirnikar R.S., Lee Y.L. Neurochem. Res. 2000; 9-10: 1439-1451.
21. Ennen C.S., Huisman T.A., Savage W.J., Northington F.J., Jennings J.M., Everett A.D., Graham E.M. Am J Obstet Gynecol. 2011; 205 (3): 251-257.
22. Garcia-Alix A., Cabanas F., Pellicer A., Hernanz A., Stiris T.A., Quero J. Pediatrics. 1994; 93: 234-240.
23. Greishen G. Biol. Neonate. 1992; 62: 243-247.
24. Kamphuis W., Mamber C., Moeton M., Kooijman L., Sluijs J.A., Jansen A.H., Verveer M., de Groot L.R., Smith V.D., Rangarajan S., Rodríguez J.J., Orre M., Hol E.M. PLoS One. 2012; 7 (8): e42823.
25. Levene M. Biol Neonate. 1992; 62: 248-251.
26. Middeldorp J., Hol E.M. Prog Neurobiol. 2011; 93 (3): 421-443.
27. Nagdyman N., Kömen W., Ko H., Muller C., Obladen M. Pediatric Research. 2001; 49 (4): 133-139.
28. Ngankam L., Kazantseva N.V., Gerasimova M.M. Zh Nevrol Psikhiatr Im S S Korsakova. 2011; 111 (7): 61-65.
29. Oh S.H., Lee J.G., Na S.J., Park J.H., Choi Y.C., Kim W.J. Arch. Neurol. 2003; 60 (1): 37-41.
30. Palmio J., Huuhka M., Laine S., Huhtala H., Peltola J., Leinonen E., Suhonen J., Keränen T. Psychiatry Res. 2010; 177 (1-2): 97-100.
31. Paulus W. Acta Neuropathol. 2009; 118 (5): 603-604.
32. Rai A., Maurya S.K., Sharma R., Ali S. Toxicol Mech Methods. 2012; DOI: 10.3109/15376516.2012.721809.
33. Ramaswamy V., Horton J., Vandermeer B., Buscemi N., Miller S., Yager J. Pediatr Neurol. 2009; 40 (3): 215-226.
34. Schiff L., Hadker N., Weiser S., Rausch C. Mol Diagn Ther. 2012; 16 (2): 79-92.
35. Sehba F.A., Hou J., Pluta R.M., Zhang J.H. Prog Neurobiol. 2012; 97 (1): 14-37.
36. Sehba F.A., Pluta R.M., Zhang J.H. Mol Neurobiol. 2011; 43 (1): 27-40.
37. Shinozaki K., Oda S., Sadahiro T., Nakamura M., Abe R., Nakada T.A., Nomura F., Nakanishi K., Kitamura N., Hirasawa H. Resuscitation. 2009; 80 (8): 870-875.
38. Siegel G., Schratt G. Identification of novel microRNA regulatory proteins in neurons using RNAi-based screening. 2012. http://www.sfn.org/siteobjects/published/3Schratt.pdf. Accessed: 13.02.2012.
39. Steinacker P., Aitken A., Otto M. Semin Cell Dev Biol. 2011; 22 (7): 696-704.
40. Streitbürger D.P., Arelin K., Kratzsch J., Thiery .J, Steiner J., Villringer A., Mueller K., Schroeter M.L. PLoS One. 2012; 7(8): e43284.
41. Thomberg E., Thiringer K., Hagberg H., Kjellmer I. Arch. Dis. Child. Fetal. Neonatal. 1995; 72: 39-42.
42. van den Berge S.A., Middeldorp J., Zhang C.E., Curtis M.A., Leonard B.W., Mastroeni D., Voorn P., van de Berg W.D., Huitinga I., Hol E.M. Aging Cell. 2010; 9 (3): 313-326.
43. Volpe J.J. Neurology of the Newborn. Saunders Elsevier. 2008; p. 1120.
For citation:
Blinov D.V. PROTEIN MARKERS OF THE HYPOXIC-ISCHEMIC INJURY OF THE CENTRAL NERVOUS SYSTEM IN THE PERINATAL PERIOD. Obstetrics, Gynecology and Reproduction. 2016;10(2):55-63. (In Russ.) https://doi.org/10.17749/2313-7347.2016.10.2.055-063
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