THE MECHANISMS OF SECONDARY OVARIAN FAILURE AT OPERATIONS ON THE PELVIC ORGANS (EXPERIMENTAL STUDY)
Abstract
Objective - to establish a hysterectomy role in formation of secondary ovarian failure and to estimate possibility of application of DHEA. Materials and Methods. Sexually mature female rats outbred Wistar. Main group: 1a - hysterectomy + salpingectomy (n=24), Ib - hysterectomy (n=6), Ic - salpingectomy (n=6). Group of comparison - hysterectomy + salpingectomy + DHEA for 4 weeks after surgery (n=12). Intact control (n=12). Enzyme immunoassay serum concentrations anti-Müllerian hormone (AMH) and follicle stimulating (FSH) hormone, testosterone (T). Results. Shows statistically significant reductions AMH after operation at all time experience (2-10-30-40 days), increased FSH, only the end of the experiment (day 40), only the reduction of T by 10 day of the experiment compared to the control. Hysterectomy was not significantly altering the concentration of AMH, FSH and T on day 10. Salpingectomy significantly reduced the concentration of AMH and T, and has no effect on FSH. Use of DHEA 1 mg / day for 28 days did not alter the concentrations of FSH and T significantly increases AMH compared to animals after surgery, but still significantly lower than that in the control. Conclusion. Isolated hysterectomy does not substantially affect the functional ovarian reserve. Isolated salpingectomy, and also the combined operation volume (hysterectomy + salpingectomy) result in secondary ovarian failure. Effect of DHEA on the function of the gonads (AMH) remains uncertain.
About the Authors
I. A. Petrov
Siberian State Medical University
Russian Federation
PhD, intramural doctoral student in Obstetrics and Gynecology
Russian Federation
PhD, intramural doctoral student in Obstetrics and Gynecology
O. A. Tihonovskaja
Siberian State Medical University
Russian Federation
MD, professor of obstetrics and gynecology
Russian Federation
MD, professor of obstetrics and gynecology
I. I. Kuprijanova
Siberian State Medical University
Russian Federation
Postgraduate of the Department of Histology, Cytology and Embryology
Russian Federation
Postgraduate of the Department of Histology, Cytology and Embryology
A. O. Okorokov
Siberian State Medical University
Russian Federation
PhD, Department of Obstetrics and Gynecology
Russian Federation
PhD, Department of Obstetrics and Gynecology
S. V. Logvinov
Siberian State Medical University
Russian Federation
MD, Professor, Vice-Rector for Academic Affairs, Head. Department of Histology, Cytology and Embryology
Russian Federation
MD, Professor, Vice-Rector for Academic Affairs, Head. Department of Histology, Cytology and Embryology
M. S. Petrova
Siberian State Medical University
Russian Federation
MD, Associate Professor of Obstetrics and Gynecology
Russian Federation
MD, Associate Professor of Obstetrics and Gynecology
L. A. Agarkova
Research Institute for Obstetrics, Gynecology and Perinatology
Russian Federation
MD, professor, honored doctor of the Russian Federation, Director
Russian Federation
MD, professor, honored doctor of the Russian Federation, Director
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For citations:
Petrov I.A., Tihonovskaja O.A., Kuprijanova I.I., Okorokov A.O., Logvinov S.V., Petrova M.S., Agarkova L.A. THE MECHANISMS OF SECONDARY OVARIAN FAILURE AT OPERATIONS ON THE PELVIC ORGANS (EXPERIMENTAL STUDY). Obstetrics, Gynecology and Reproduction. 2015;9(4):6-17. (In Russ.) https://doi.org/10.17749/2070-4968.2015.9.4.006-017
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