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Characteristic of spermogram parameters in men with reproductive pathology in age-related aspect

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Introduction. The prevalence of andrological diseases among adolescents and young adults resulting in lowered reproductive potential has been noted to progressively increase. At the same time, the number of couples starting to manage reproductive issues after 35–40 years of age highlighting the onset of male androgen deficiency continues to rise. Therefore, the analysis of spermogram as the key element in assessing male reproductive potential is better to conduct at different age periods of man's life.

Aim: to compare spermogram parameters in different age groups of patients with reproductive pathology.

Materials and Мethods. The analysis of spermograms in adolescents with left-sided grade II–III varicocele aged 17 years and in infertile males aged 22–48 years was performed. Semen analysis was conducted in accordance with the standards of the 5 th edition of the World Health Organization and included the following parameters: semen volume (ml), sperm concentration (million/ml), total sperm count (million), acidity, viscosity, progressive motility, total motility, viability, morphology, detected mucus, leukocytes, amyloid bodies, lecithin grains as well as sperm aggregation and agglutination. The stained preparations were used to assess the morphology of spermatozoa and spermatogenesis cells. According to the spermogram data obtained, the following conclusions were drawn: normozoospermia, oligozoospermia, asthenozoospermia, teratozoospermia. Statistical analysis was performed by using Statistica 10.0 software (StatSoft Inc., USA). The normality distribution was assessed using the χ2 test. Quantitative parameters were presented as arithmetic means and standard deviations (M ± SD). Assessing significance of differences was performed by using the Student's t-test, whereas inter-parameter correlation relations were analyzed by using the linear Pearson's correlation coefficient. A significance level between inter-group parameters was set at p < 0.05.

Results. It was found that adolescents with varicocele vs. adult men had significantly decreased ejaculate volume. In particular, the average ejaculate volume in adolescents and adult men was 2.32 ± 1.22 ml and 3.50 ± 1.44 ml, respectively, so that the larger number of young patients were noted to have ejaculate volume below 1.5 ml. Compared to young subjects, aged patients had decreased sperm concentration (35.88 ± 25.74 versus 72.20 ± 49.32 million/ml) and total sperm count (120.58 ± 91.72 versus 173.07 ± 163.92 million). Young patients were found to have significantly superior data in all categories of sperm motility, whereas infertile men were diagnosed with impaired sperm motility. In particular, adolescents were featured with the average number of spermatozoa displaying fast and slow translational movement comprising 17.12 ± 11.04 % and 29.30 ± 12.29 %, respectively, the proportion of progressive motility spermatozoa was 46.20 ± 19.82 %. In contrast, similar parameters in adult men were 5.10 ± 6.36 %, 19.80 ± 9.61 %, and 24.95 ± 11.23 %, respectively. In infertile men prevalence of lacked spermatozoa with rapid forward movement was 46 (46.0 %), in adolescents – 8 (8.6%), whereas rate of immotile spermatozoa in infertile men, on average, accounted for 53.10 ± 14.56 %, in adolescents – 34.40 ± 21.83 %. In addition, adolescents with varicocele had significantly fewer spermatozoa with normal morphology – 14.14 ± 8.06 % (in adult men – 30.08 ± 17.94 %), there were more abundant defects in the sperm head – 58.01 ± 12.43 % (in men – 48.83 ± 18.95 %) and flagella – 17.24 ± 6.31 % (in men – 10.29 ± 6.21 %). The data obtained showed that adolescents were more often diagnosed with normozoospermia – in 49 (52.7 %) cases, in infertile men – in 12 (12.0 %) cases, whereas in aged men asthenozoospermia was detected in 82 (82.0 %) cases, in adolescents – 5 (5.4 %) cases.

Conclusion. The abnormalities in the spermogram revealed in adolescents may be associated with unestablished spermatogenesis. Normozoospermia more common in adolescents with varicocele may evidence about preserved reproductive potential. Impaired sperm motility in aged patients seems to be related to the formation of oxidative stress and damage to spermatozoa by reactive oxygen species due to combined age-related changes, cumulation of the negative effects of environmental and lifestyle factors, as well as comorbidities.

About the Authors

S. V. Pichugova
Institute of Immunology and Physiology, Ural Branch of Russian Academy of Sciences; Sverdlovsk region «Clinical and Diagnostic Center Ekaterinburg city»
Russian Federation

Svetlana V. Pichugova – MD, PhD, Senior Researcher, Laboratory of Immunopathophysiology; Doctor of the Highest Category, Laboratory of Electronic Microscopy

106 Pervomaiskaya Str., Еkaterinburg 620049
78В Str. 8 Marta, Ekaterinburg 620144

V. A. Chereshnev
Institute of Immunology and Physiology, Ural Branch of Russian Academy of Sciences
Russian Federation

Valeriy A. Chereshnev – MD, Dr Sci Med, Professor, Academician of RAS, Chief Researcher, Laboratory of Immunophysiology and Immunopharmacology. Scopus Author ID: 6602886513. Researcher ID: P-7704-2017

106 Pervomaiskaya Str., Еkaterinburg 620049

Ya. B. Beikin
Institute of Immunology and Physiology, Ural Branch of Russian Academy of Sciences; Sverdlovsk region «Clinical and Diagnostic Center Ekaterinburg city»
Russian Federation

Yakov B. Beikin – MD, Dr Sci Med, Professor, Head of the Laboratory of Immunopathophysiology; Chief Physician; Honored Doctor of the Russian Federation

106 Pervomaiskaya Str., Еkaterinburg 620049
78В Str. 8 Marta, Ekaterinburg 620144


1. Barati E., Nikzad H., Karimian M. Oxidative stress and male infertility: current knowledge of pathophysiology and role of antioxidant therapy in disease management. Cell Mol Life Sci. 2020;77(1):93–113.

2. Shiraishi K., Matsuyama H. Effects of medical comorbidity on male infertility and comorbidity treatment on spermatogenesis. Fertil Steril. 2018;110(6):1006–11.e2.

3. Potekhina E.S., Mikhaylyuk E.V., Nepomnyaschikh A.S. Spermogram as an instrument for assessing male fertility. [Spermogramma kak instrument ocenki muzhskoj fertil'nosti]. Nauchnoe obozrenie. 2020;(1):11–4. (In Russ.).

4. Zhukov O.B., Evdokimov V.V., Bragina E.E. Improvement of quality of life and morphofunctional characteristics of spermatozoa in men with chronic nonbacterial prostatitis and in a program of preconception preparation to fatherhood. [Uluchshenie kachestva zhizni i morfofunkcional'nyh harakteristik spermatozoidov u muzhchin s hronicheskim abakterial'nym prostatitom i programmy pregravidarnoj podgotovki k otcovstvu]. Andrologiya i genital'naya hirurgiya. 2017;18(1):102–8. (In Russ.).

5. Avadieva N.E. The use of DNA semen fragmentation in andrological practice. [Primenenie DNK fragmentacii spermy v andrologicheskoj praktike]. Vestnik urologii. 2019;1(7):7–11. (In Russ.).

6. Barratt C.L.R., Björndahl L., De Jonge C.J. et al. The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance-challenges and future research opportunities. Hum Reprod Update. 2017;23(6):660–80.

7. Busetto G.M., Del Giudice F., Virmani A. et al. Body mass index and age correlate with antioxidant supplementation effects on sperm quality: Post hoc analyses from a double-blind placebo-controlled trial. Andrologia. 2020;52(3):e13523.

8. Agarwal A., Rana M., Qiu E. et al. Role of oxidative stress, infection and inflammation in male infertility. Andrologia. 2018;50(11):e13126.

9. Kuznetsova N.N., Shamin M.V., Farbirovich V.Ya. et al. Examination of reproductive potential of Kuzbass young men and role of chronic inflammation in reproductive tract as factor of ejaculate’s fertility decrease. [Ocenka reproduktivnogo potenciala molodyh muzhchin Kuzbassa i rol' hronicheskogo vospaleniya organov reproduktivnogo trakta kak faktora snizheniya fertil'nosti eyakulyata]. Omskij nauchnyj vestnik. 2015;(1):71–3. (In Russ.).

10. Ovchinnikov R.I. Gamidov S.I., Popova A.Yu. et al. The causes of reproductive losses in men are sperm DNA fragmentation. [Prichiny reproduktivnyh poter' u muzhchin – fragmentaciya DNK spermatozoidov]. RMZh. 2015;23(11):634–8. (In Russ.).

11. Esteves S.C., Agarwal A. Afterword to varicocele and male infertility: current concepts and future perspectives. Asian J Androl. 2016;18(2):319–22.

12. Alsaikhan B., Alrabeeah K., Delouya G., Zini A. Epidemiology of varicocele. Asian J Androl. 2016;18(2):179–81.

13. Jensen C.F.S., Østergren P., Dupree J.M. et al. Varicocele and male infertility. Nat Rev Urol. 2017;14(9):523–33.

14. Majzoub A., Esteves S.C., Gosálvez J., Agarwal A. Specialized sperm function tests in varicocele and the future of andrology laboratory. Asian J Androl. 2016;18(2):205–12.

15. Kathrins M. Historical investigations into varicocele pathophysiology and sperm migration. Fertil Steril. 2018;109(1):75–6.

16. Kruger T. Critical appraisal of conventional semen analysis in the context of varicocele. Asian J Androl. 2016;18(2):202–4.

17. Santana V.P., Miranda-Furtado C.L., de Oliveira-Gennaro F.G., Dos Reis R.M. Genetics and epigenetics of varicocele pathophysiology: an overview. J Assist Reprod Genet. 2017;34(7):839–47.

18. Brannigan R.E. Introduction: Varicoceles: a contemporary perspective. Fertil Steril. 2017;108(3):361–3.

19. Efremov E.A., Kasatonova E.V., Melnik Ya.I., Simakov V.V. Advanced paternal age: review of ejaculate damaging mechanisms, risks and strategies to overcome them. [Pozdnee otcovstvo: obzor povrezhdayushchih eyakulyat mekhanizmov, riskov i strategij ih preodoleniya]. Effektivnaya farmakoterapiya. 2016;(11):16–33. (In Russ.).

20. Sulima A.N., Litvinov V.V., Klimenko P.M. et al. Features of male infertility as the only factor of infertility of a married couple in the ART clinic. [Osobennosti muzhskoj infertil'nosti kak edinstvennogo faktora besplodiya supruzheskoj pary v klinike VRT]. Eksperimental'naya i klinicheskaya urologiya. 2019;(4):68–73. (In Russ.).

21. Kirilenko E.A., Onopko V.F. Oxidative stress and male fertility: modern view on the problem. [Okislitel'nyj stress i muzhskaya fertil'nost': sovremennyj vzglyad na problem]. Byulleten' VSNC SO RAMN. 2017;2(2):102–8. (In Russ.).

22. Osadchuk L.V., Kleschev M.A., Tipisova E.V., Osadchuk A.V. Parameters of spermatogenesis and hormonal and metabolic statuses in men of different age groups from Northern European Russia. [Pokazateli spermatogeneza, gormonal'nogo i metabolicheskogo statusa u muzhchin raznyh vozrastnyh grupp na Evropejskom severe Rossii]. Fiziologiya cheloveka. 2019;3(45):107–14. (In Russ.).

23. Shridharani A., Owen R.C., Elkelany O.O., Kim E.D. The significance of clinical practice guidelines on adult varicocele detection and management. Asian J Androl. 2016;18(2):269–75.

24. Kumar M., Selvam P., Agarwal A. Sperm and seminal plasma proteomics: molecular changes associated with varicocele-mediated male infertility. World J Mens Health. 2020;38(4):472–83.

25. Oliva A., Multigner L. Chronic epididymitis and grade III varicocele and their associations with semen characteristics in men consulting for couple infertility. Asian J Androl. 2018;20(4):360–5.

26. WHO laboratory manual for the examination and processing of human semen. 5th ed. [Rukovodstvo VOZ po issledovaniyu i obrabotke eyakulyata cheloveka. 5-e izdanie. Per. s angl. N.P. Makarova, pod red. L.F. Kurilo]. Moscow: Izd-vo Kapital print, 2012. 305 p. (In Russ.).

27. Shmidt A.A., Zamyatin S.A., Gonchar I.S., Korovin A.E. Risk factors for the development of male infertility. [Faktory riska razvitiya muzhskoj infertil'nosti]. Klinicheskaya patofiziologiya. 2019;25(4):56–60. (In Russ.).

28. Belardin L.B., Del Giudice P.T., Camargo M. et al. Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele. J Assist Reprod Genet. 2016;33(12):1657–64.

29. Glassberg K.I. My indications for treatment of the adolescent varicocele (and why?). Transl Androl Urol. 2014;3(4):402–12.

30. Cho C.L., Esteves S.C., Agarwal A. Novel insights into the pathophysiology of varicocele and its association with reactive oxygen species and sperm DNA fragmentation. Asian J Androl. 2016;18(2):186–93.

31. Zhang Y., Ma T., Su Z. et al. Varicoceles affect semen quality of infertile men in Southern China: A cross-sectional study of 5447 cases. Medicine (Baltimore). 2017;96(31):e7707.

32. Santana V.P., Miranda-Furtado C.L., Pedroso D.C.C. et al. The relationship among sperm global DNA methylation, telomere length, and DNA fragmentation in varicocele: a cross-sectional study of 20 cases. Syst Biol Reprod Med. 2019;65(2):95–104.

33. Andreev R.Yu., Rasner P.I., Malkhasyan V.A. et al. Varicocele – what we know about him? [Varikocele – chto nam o nem izvestno?] Moskovskij hirurgicheskij zhurnal. 2019;(5):24–31. (In Russ.).

34. Esteves S.C. Novel concepts in male factor infertility: clinical and laboratory perspectives. J Assist Reprod Genet. 2016;33(10):1319–35.

35. Martins A.D., Agarwal A. Oxidation reduction potential: a new biomarker of male infertility. Panminerva Med. 2019;61(2):108–17.

36. Tahamtan S., Tavalaee M., Izadi T. et al. Reduced sperm telomere length in individuals with varicocele is associated with reduced genomic integrity. Sci Rep. 2019;9(1):4336.

37. Pallotti F., Paoli D., Carlini T. et al. Varicocele and semen quality: a retrospective case-control study of 4230 patients from a single centre. J Endocrinol Invest. 2018;41(2):185–92.

38. Bazhenov I.V., Filippova E.S. Role of oxidative stress in the male infertility pathogenesis. [Rol' okislitel'nogo stressa v patogeneze muzhskogo besplodiya]. Effektivnaya farmakoterapiya. 2018;(29):50–8. (In Russ.).

39. Kalinchenko S.Yu., Tyuzikov I.A. Oxidative stress and male infertility – XXI-review century interrelated pandemics. Current pharmacotherapeutic opportunities of pathogenetic correction of spermatogenesis disorders with L-carnitine/acetyl-L-carnitine. [Okislitel'nyj stress i muzhskoe besplodie – vzaimosvyazannye pandemii XXI v. Sovremennye farmakoterapevticheskie vozmozhnosti patogeneticheskoj korrekcii narushenij spermatogeneza preparatami L-karnitina/acetil-L-karnitina]. Effektivnaya farmakoterapiya. 2017;(22):6–19. (In Russ.).

40. Korneev I.A. Male infertility treatments: clinical overview. [Terapiya muzhskogo besplodiya: analiz issledovanij]. Medicinskij sovet. 2019;(13):99–104. (In Russ.).


For citation:

Pichugova S.V., Chereshnev V.A., Beikin Y.B. Characteristic of spermogram parameters in men with reproductive pathology in age-related aspect. Obstetrics, Gynecology and Reproduction. 2021;15(6):715-725. (In Russ.)

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