akusherstvoObstetrics, Gynecology and ReproductionАкушерство, Гинекология и Репродукция2313-73472500-3194IRBIS LLC10.17749/2070-4968.2015.9.4.006-017akusherstvo-176Research ArticleОRIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИTHE MECHANISMS OF SECONDARY OVARIAN FAILURE AT OPERATIONS ON THE PELVIC ORGANS (EXPERIMENTAL STUDY)МЕХАНИЗМЫ ВТОРИЧНОЙ НЕДОСТАТОЧНОСТИ ЯИЧНИКОВ ПРИ ОПЕРАЦИЯХНА ОРГАНАХ МАЛОГО ТАЗА (ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ)ПетровИ. А.PetrovI. A.

к.м.н., ассистент и очный докторант кафедры акушерства и гинекологии

PhD, intramural doctoral student in Obstetrics and Gynecology

obgynsib@gmail.comТихоновскаяО. А.TihonovskajaO. A.

д.м.н., профессор кафедры акушерства и гинекологии

MD, professor of obstetrics and gynecology

КуприяноваИ. И.KuprijanovaI. I.

аспирант кафедры гистологии, эмбриологии и цитологии

Postgraduate of the Department of Histology, Cytology and Embryology

ОкороковА. О.OkorokovA. O.

к.м.н., ассистент кафедры акушерства и гинекологии

PhD, Department of Obstetrics and Gynecology

ЛогвиновС. В.LogvinovS. V.

д.м.н., профессор, проректор по учебной работе, зав. кафедрой гистологии, эмбриологии и цитологии

MD, Professor, Vice-Rector for Academic Affairs, Head. Department of Histology, Cytology and Embryology

ПетроваМ. С.PetrovaM. S.

к.м.н., доцент кафедры акушерства и гинекологии

MD, Associate Professor of Obstetrics and Gynecology

АгарковаЛ. А.AgarkovaL. A.

д.м.н., профессор, заслуженный врач РФ, директор

MD, professor, honored doctor of the Russian Federation, Director

ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России, г. ТомскРоссияSiberian State Medical UniversityRussian FederationФГБУ «Научно-исследовательский институт акушерства, гинекологии и перинатологии» Сибирского отделения РАН, г. ТомскРоссияResearch Institute for Obstetrics, Gynecology and PerinatologyRussian Federation20152006201694617Copyright © Petrov I.A., Tihonovskaja O.A., Kuprijanova I.I., Okorokov A.O., Logvinov S.V., Petrova M.S., Agarkova L.A., 20162016Петров И.А., Тихоновская О.А., Куприянова И.И., Окороков А.О., Логвинов С.В., Петрова М.С., Агаркова Л.А.Petrov I.A., Tihonovskaja O.A., Kuprijanova I.I., Okorokov A.O., Logvinov S.V., Petrova M.S., Agarkova L.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.gynecology.su/jour/article/view/176

Objective - to establish a hysterectomy role in formation of secondary ovarian failure and to estimate possibility of application of DHEA. Materials and Methods. Sexually mature female rats outbred Wistar. Main group: 1a - hysterectomy + salpingectomy (n=24), Ib - hysterectomy (n=6), Ic - salpingectomy (n=6). Group of comparison - hysterectomy + salpingectomy + DHEA for 4 weeks after surgery (n=12). Intact control (n=12). Enzyme immunoassay serum concentrations anti-Müllerian hormone (AMH) and follicle stimulating (FSH) hormone, testosterone (T). Results. Shows statistically significant reductions AMH after operation at all time experience (2-10-30-40 days), increased FSH, only the end of the experiment (day 40), only the reduction of T by 10 day of the experiment compared to the control. Hysterectomy was not significantly altering the concentration of AMH, FSH and T on day 10. Salpingectomy significantly reduced the concentration of AMH and T, and has no effect on FSH. Use of DHEA 1 mg / day for 28 days did not alter the concentrations of FSH and T significantly increases AMH compared to animals after surgery, but still significantly lower than that in the control. Conclusion. Isolated hysterectomy does not substantially affect the functional ovarian reserve. Isolated salpingectomy, and also the combined operation volume (hysterectomy + salpingectomy) result in secondary ovarian failure. Effect of DHEA on the function of the gonads (AMH) remains uncertain.

Цель работы - установить роль гистерэктомии в формировании вторичной недостаточности яичников и оценить возможность применения DHEA. Материалы и методы. Половозрелые крысы-самки аутбредного стока Вистар. Основная группа: 1а - гистерэктомия + сальпингэктомия (n=24), Iб - гистерэктомия (n=6), Iв - сальпингэктомия (n=6). Группа сравнения - гистерэктомия + сальпингэктомия + DHEA в течение четырех недель после операции (n=12). Интактный контроль (n=12). Иммуноферментный анализ сывороточных концентраций антимюллерова (АМГ) и фолликулостимулирующего (ФСГ) гормонов, тестостерона (Т). Результаты. Установлено статистически значимое снижение АМГ после гистерэктомии и сальпингэктомии во все сроки опыта (2-10-30-40-е сут.), повышение ФСГ только к 40-м сут., снижение Т - только на 10-е сутки опыта по сравнению с контролем. Гистерэктомия значимо не изменяет концентрации АМГ, ФСГ и Т на 10-е сутки. Сальпингэктомия значимо снижает концентрации АМГ и Т и не влияет на ФСГ. Применение DHEA в дозе 1 мг/сут. в течение 28 дней не изменяет концентрации ФСГ и Т, значимо увеличивает АМГ по сравнению с таковым у животных после операции, но остается значимо ниже такового в контроле. Заключение. Изолированная гистерэктомия существенно не влияет на функциональный овариальный резерв. Изолированная сальпингэктомия, а также комбинированный объем операции (гистерэктомия + сальпингэктомия) приводят к вторичной недостаточности яичников. Эффект DHEA на функцию гонад остается сомнительным.

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The authors declare that there are no conflicts of interest present.