akusherstvoObstetrics, Gynecology and ReproductionАкушерство, Гинекология и Репродукция2313-73472500-3194IRBIS LLC10.17749/2313-7347/ob.gyn.rep.2023.413akusherstvo-1634Research ArticleОRIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИExpression profile of plasma microRNAs and target genes in patients with complicated pregnancyПрофиль экспрессии плазматических микроРНК и генов-мишеней у пациенток с осложненным течением беременностиhttps://orcid.org/0000-0002-5449-2982ПашковскийД. Г.PashkovskyD. G.

Пашковский Дмитрий Геннадьевич – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Dmitry G. Pashkovsky – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0002-5812-6779СоловьеваЕ. В.SolovievaE. V.

Соловьева Екатерина Васильевна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Ekaterina V. Solovieva – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0001-9457-757XРабадановаЦ. Р.RabadanovaTs. R.

Рабаданова Цибац Рабазановна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Tsibats R. Rabadanova – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0003-0175-1968ГорбуноваП. Т.GorbunovaP. T.

Горбунова Полина Тарасовна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Polina T. Gorbunova – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0001-8507-5797ДубоваяА. Б.DubovayaA. B.

Дубовая Анастасия Богдановна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Anastasia B. Dubovaya – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0002-0510-1324МуслимоваЭ. Р.MuslimovaE. R.

Муслимова Эмилие Рефатовна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Emilie R. Muslimova – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0001-7157-5846ХорозЭ. Э.KhorozE. E.

Хороз Эдие Эльдаровна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Edie E. Khoroz – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0003-4133-9628КарабашЗ. С.KarabashZ. S.

Карабаш Зекие Серверовна – студент

295051 Симферополь, бульвар Ленина, д. 5/7

Zekie S. Karabash – Student

5/7 Lenin Avenue, Simferopol 295051

https://orcid.org/0000-0002-1862-6816СорокинаЛ. Е.SorokinaL. E.

Сорокина Лея Евгеньевна – аспирант

115478 Москва, Каширское шоссе, 24, корп. 2

Leya E. Sorokina – MD

24 bldg. 2, Kashirskoe Highway, Moscow 115478

leya.sorokina@mail.ruИнститут «Медицинская академия имени С. И. Георгиевского», ФГАОУ ВО «Крымский федеральный университет имени В. И. Вернадского»РоссияInstitute "Georgievsky Medical Academy", Vernadsky Crimean Federal UniversityRussian FederationФГБУ «Государственный научный центр "Институт иммунологии и аллергологии" Федерального медико-биологического агентства»РоссияNational Research Center – Institute of Immunology, Federal Medical-Biological Agency of RussiaRussian Federation202324042023172231243Copyright © Pashkovsky D.G., Solovieva E.V., Rabadanova T.R., Gorbunova P.T., Dubovaya A.B., Muslimova E.R., Khoroz E.E., Karabash Z.S., Sorokina L.E., 20232023Пашковский Д.Г., Соловьева Е.В., Рабаданова Ц.Р., Горбунова П.Т., Дубовая А.Б., Муслимова Э.Р., Хороз Э.Э., Карабаш З.С., Сорокина Л.Е.Pashkovsky D.G., Solovieva E.V., Rabadanova T.R., Gorbunova P.T., Dubovaya A.B., Muslimova E.R., Khoroz E.E., Karabash Z.S., Sorokina L.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.gynecology.su/jour/article/view/1634Aim

Aim: comparative analysis of the expression profile of plasma microRNAs and target genes in patients with complicated pregnancy.

Materials and Methods

Materials and Methods. A prospective observational comparative study in parallel groups was carried out. The study included 73 women divided into three groups: the main group – 42 patients with preeclampsia (PE), the comparison group – 12 pregnant women with fetal growth retardation (FGR), the control group – 19 clinically healthy women with uncomplicated pregnancy. An examination was performed, which included the analysis of clinical characteristics and the study of microRNA expression in blood plasma using the real-time polymerase chain reaction.

Results

Results. MicroRNA hsa-miR-210-5p and hsa-miR-1972 were not identified in any plasma sample. Analyzing plasma microRNAs in group of women with PE showed significant changes in the expression levels of hsa-miR-517a-3p (p = 0.025), hsa-miR-517c-3p (p = 0.036), hsa-miR-574-5p (p = 0.015), hsa-miR-517a-3p (p < 0.001) and an increase in miR-20a-5p (p = 0.046) compared to control group. No significant differences were found in the miRNA expression profile in group of women with FGR compared to control group. Assessing an influence of the studied microRNAs on regulatory signaling pathways allowed to establish that hsa-miR-miR-146a-5p, -181a-5p, -210-3p, -517a-3p, -517c-3p, -574-3p, -574-5p, -1304-5p are potential regulators of the reaction cascades involved in the PE pathogenesis.

Conclusion

Conclusion. The changes revealed in the circulating blood plasma miRNA level indicate the presence of specific transcriptomic alterations during complicated course of pregnancy.

Цель

Цель: сравнительный анализ профиля экспрессии плазматических микроРНК и генов-мишеней у пациенток с осложненным течением беременности.

Материалы и методы

Материалы и методы. Проведено проспективное наблюдательное сравнительное исследование в параллельных группах. В исследование включены 73 женщины, разделенные на 3 группы: основная группа – 42 пациентки с преэклампсией (ПЭ), группа сравнения – 12 беременных с задержкой роста плода (ЗРП), контрольная группа – 19 клинически здоровых женщин с неосложненным течением беременности. Выполнено обследование, включавшее анализ клинических характеристик и изучение экспрессии микроРНК в плазме крови с помощью метода полимеразной цепной реакции в режиме реального времени.

Результаты

Результаты. МикроРНК hsa-miR-210-5p и hsa-miR-1972 не были идентифицированы ни в одном образце плазмы. Анализ плазменных микроРНК в основной группе (женщины с ПЭ) показал статистически значимые изменения уровней экспрессии hsa-miR-517a-3p (p = 0,025), hsa-miR-517с-3p (p = 0,036), hsa-miR-574-5p (p = 0,015), hsa-miR-517a-3p (p < 0,001) и повышение miR-20a-5p (р = 0,046) по сравнению с контрольной группой. Значимых различий в профиле экспрессии микроРНК в группе женщин с ЗРП по сравнению с контрольной группой выявлено не было. Проведенная оценка влияния изученных микроРНК на регуляторные сигнальные пути позволила установить, что hsa-miR-miR-146a-5p, -181a-5p, -210-3p, -517a-3p, -517с-3p, -574-3p, -574-5p, -1304-5p являются потенциальными регуляторами каскадов реакций, вовлеченных в патогенез ПЭ.

Заключение

Заключение. Выявленные изменения содержания циркулирующих в плазме крови микроРНК свидетельствуют о наличии специфических молекулярных изменений на уровне транскриптома при осложнённом течении беременности.

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The authors declare that there are no conflicts of interest present.